Ames II Mutagenicity Assay Technical Documentation
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Ames II Mutagenicity Assay Technical Documentation
Statistical features of a base-specific Salmonella mutagenicity assay are considered in detail, following up on a previous Ž . report comparing responses of base-specific Salmonella Ames IIe strains with those of traditional tester strains. In addition to using different Salmonella strains, the new procedure also differs in that it is performed as a microwell fluctuation test, as opposed to the...
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Mebudipine is a new dihydropyridine calcium channel blocker, synthesized in our laboratory, for treatment of hypertension. It has shown a better efficacy than other drugs in this group. For assessing the risks of this drug, certain safety tests in the preclinical stage have been performed. In this study mutagenic effect of mebudipine was evaluated using Ames assay that could assess the mutageni...
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The Ames II Salmonella mutagenicity assay procedure was used to test 71 chemicals, and the results were compared with those from the traditional Ames Salmonella test using the NTP database as the reference. All Ames II tests were performed using a fluctuation procedure in microplate format, using TAMix for the detection of base pair substitutions and TA98 to detect frameshift mutations. There w...
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(7) Claxton, L., Houk, V.S., Monteith, L.G., Myers, L.E., Hughes, T.J., ‘‘Assessing the use of known mutagens to calibrate the Salmonella typhimurium mutagenicity assay: I. Without exogenous activation.’’ Mutation Research 253:137–147 (1991). (8) Claxton, L., Houk, V.S., Warner, J.R., Myers, L.E., Hughes, T.J., ‘‘Assessing the use of known mutagens to calibrate the Salmonella typhimurium mutage...
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